TRANSFORMATION OF ACTINOMYCES SPP BY A GRAM-NEGATIVE BROAD-HOST-RANGEPLASMID

The gram-negative broad-host-range vector pJRD215 was transferred by e lectroporation into strains of Actinomyces viscosus or Actinomyces nae slundii at efficiencies which ranged from 10(2) to 10(7) transformants per mu g of plasmid DNA. The Actinomyces transformants expressed pJRD 215-encoded resistance to kanamycin and streptomycin. Moreover, the tr ansforming plasmid DNA had not undergone any deletions or rearrangemen ts, nor had it integrated into the genomes of these strains.