Cladistic relationships among Gesneriaceae and related families are in
vestigated using ndhF sequence data, to establish the affinity of thre
e genera whose familial placement was uncertain on the basis of morpho
logical evidence alone. A first analysis, including 18 outgroup specie
s from the Buddlejaceae, Scrophulariaceae, Bignoniaceae, and Acanthace
ae resulted in eleven most-parsimonious trees of 5740 steps each. Rece
nt classifications had placed Cyrtandromoea in Scrophulariaceae or Ges
neriaceae, Titanotrichum as a monogeneric tribe in Gesneriaceae, and S
anango in either Scrophulariaceae, Gesneriaceae, Loganiaceae, or Buddl
ejaceae. Sequence data from the chloroplast ndhF gene indicate that th
ey are Gesneriaceae: Cyrtandromoea belongs to the Klugieae, the monoge
neric Titanotricheae are perhaps sister to the remainder of the Cyrtan
droideae, and Sanango is placed in the Gesnerieae as was predicted by
recent morphological and chemical analyses.