NOVEL SULFONE-BASED THIOPHILIC LIGANDS FOR THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC PURIFICATION OF ANTIBODIES

Novel thiophilic ligands based on sulfone structures were synthesized for the fast high-performance liquid chromatographic (HPLC) purificati on of antibodies. In order to better characterize these new structures , affinity constants were measured as well as the influence of pH and salt on adsorption and elution. Efficient and fast purification of ant ibodies from ascites and serum was achieved. The purification of antib odies from cell culture supernatant proved difficult due the indicator pheno-red of the growth media. Two thiophilic structures turned out t o be protein adsorbents, which adsorbed all proteins loaded and from w hich no protein could be eluted under normal conditions.