5-HT2A RECEPTOR-MEDIATED OUTWARD CURRENT IN C6 GLIOMA-CELLS IS MIMICKED BY INTRACELLULAR IP3 RELEASE

Citation
Jt. Bartrup et Nr. Newberry, 5-HT2A RECEPTOR-MEDIATED OUTWARD CURRENT IN C6 GLIOMA-CELLS IS MIMICKED BY INTRACELLULAR IP3 RELEASE, NeuroReport, 5(10), 1994, pp. 1245-1248
Citations number
16
Categorie Soggetti
Neurosciences
Journal title
ISSN journal
09594965
Volume
5
Issue
10
Year of publication
1994
Pages
1245 - 1248
Database
ISI
SICI code
0959-4965(1994)5:10<1245:5ROCIC>2.0.ZU;2-5
Abstract
THE C6 glioma cell line possesses 5-HT2A receptors that have been show n to increase intracellular calcium levels. We have studied the electr ophysiological response of these cells to 5-HT using the whole-cell re cording method. Under voltage-clamp, 5-hydroxytryptamine (5-HT) produc ed an outward current in these cells which was inhibited by extracellu larly applied ketanserin and spiperone and by EGTA (10 mM) in the reco rding electrode. The 5-HT induced response could be mimicked by intrac ellular photolytic release of inositol (1,4,5) trisphosphate (IP3) fro m caged molecules. The reversal potentials for the IP3- and 5-HT-induc ed responses were closely matched. The data indicates that the outward current is likely to be mediated by 5-HT2A receptors stimulating IP3 production which increases intracellular calcium leading to the openin g of calcium-activated potassium channels.