DETERMINATION OF CHROMOSOME SIZE AND NUMBER OF RRN LOCI IN LACTOBACILLUS-PLANTARUM BY PULSED-FIELD GEL-ELECTROPHORESIS

Authors
CHEVALLIER B HUBERT JC KAMMERER B
Citation
B. Chevallier et al., DETERMINATION OF CHROMOSOME SIZE AND NUMBER OF RRN LOCI IN LACTOBACILLUS-PLANTARUM BY PULSED-FIELD GEL-ELECTROPHORESIS, FEMS microbiology letters, 120(1-2), 1994, pp. 51-56
Citations number
19
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
120
Issue
1-2
Year of publication
1994
Pages
51 - 56
Database
ISI
SICI code
0378-1097(1994)120:1-2<51:DOCSAN>2.0.ZU;2-G
Abstract
The size of the Lactobacillus plantarum CCM 1904 chromosome was determ inated by pulse-field gel electrophoresis. It was found to be 3.3-3.4 Mb using SfiI or AscI restriction endonucleases, compared to 3-4 Mb fo und for the other L. plantarum strains tested. L. plantarum CCM 1904 5 S rDNA was cloned by polymerase chain reaction, sequenced, and used as a probe to characterize strains. At least five rrn loci were found. T he pulsed-field gel electrophoresis macrorestriction patterns were str ain-specific, while the rDNA restriction hybridization patterns were s pecies-specific.