FRACTIONATION OF SUBCELLULAR MEMBRANES OF THE SECRETORY PATHWAY FROM THE PEROXIDASE-PRODUCING WHITE-ROT FUNGUS PHANEROCHAETE-CHRYSOSPORIUM

Mycelia from the basidiomycete Phanerochaete chrysosporium, producing lignin and manganese peroxidases, were homogenized and fractionated on a sucrose gradient. The main subcellular fungal membrane fractions we re successfully separated. Lipid composition analyses of the isolated membranes as well as associated marker enzymes distribution gave evide nce to similarities with membranes originating from plants. Lignin and manganese peroxidases were investigated by immunodetection in subcell ular fractions. Our results show that lignin and manganese peroxidases are mainly associated with Golgi apparatus vesicles and, to a lesser extent, with endoplasmic reticulum and light density vesicles, but not with plasma membranes.