REAL-TIME MEASUREMENT OF GENE-EXPRESSION IN LIVING ENDOCRINE-CELLS

Authors
FRAWLEY LS FAUGHT WJ NICHOLSON J MOOMAW B
Citation
Ls. Frawley et al., REAL-TIME MEASUREMENT OF GENE-EXPRESSION IN LIVING ENDOCRINE-CELLS, Endocrinology, 135(1), 1994, pp. 468-471
Citations number
6
Categorie Soggetti
Endocrynology & Metabolism
Journal title
Endocrinology → ACNP
ISSN journal
00137227
Volume
135
Issue
1
Year of publication
1994
Pages
468 - 471
Database
ISI
SICI code
0013-7227(1994)135:1<468:RMOGIL>2.0.ZU;2-N
Abstract
The mechanisms underlying hormonal gene expression are complex, and ke y regulatory events are often temporally separated. Our understanding of these dynamic processes has been hindered in part by the lack of a method suitable for making dynamic measurements of gene expression. In an attempt to develop such a system, we modified conventional lucifer ase reporter technology to enable real time measurement of gene expres sion in living endocrine cells.