Je. Baggott et al., DIFFERENCES IN METHOTREXATE AND 7-HYDROXYMETHOTREXATE INHIBITION OF FOLATE-DEPENDENT ENZYMES OF PURINE NUCLEOTIDE BIOSYNTHESIS, Biochemical journal, 300, 1994, pp. 627-629
7-Hydroxymethotrexate (7-OH-MTX) is the major and, frequently the only
, pteridine metabolite found in bone-marrow aspirates of patients chro
nically treated with low-dose oral methotrexate (MTX) [Sonneveld, Schu
ltz, Nooter and Hahlen (1986) Cancer Chemother. Pharmacol. 18, 111-116
]. The K-i values for MTX and 7-OH-MTX for avian liver 5-aminoimidazol
e-4-carboxamide ribonucleotide transformylase differ by 4.5-fold in fa
vour of 7-OH-MTX as the better inhibitor, while Ki values for avian li
ver glycinamide ribonucleotide transformylase differ by 1.9-fold favou
ring MTX as the better inhibitor. Thus 7-OH-MTX possesses a different
enzyme-inhibiting repertoire from its parent drug and this information
may be useful in explaining the mechanism of action of low-dose MTX t
herapies used to treat autoimmune disease.