TXK, A NOVEL HUMAN TYROSINE KINASE EXPRESSED IN T-CELLS SHARES SEQUENCE IDENTITY WITH TEC FAMILY KINASES AND MAPS TO 4P12

A gene for a novel, putative cytoplasmic tyrosine kinase, TXK has been isolated from a human peripheral blood cDNA library. The complete nuc leotide sequence of the cDNA indicates that it is related mast closely to EMT, a tyrosine kinase of T cells and to the B-cell tyrosine kinas e Btk, which is mutated in X-linked agammaglobulinemia (XLA) in humans and X-linked immunodeficiency disease (XID) in mouse. TXK, like BTK, is a member of the Tec sub-family of Src-type (non-receptor) tyrosine kinases. Like similar Tec subfamily members, and unlike the other Src kinases, TXK lacks both the N-terminal myristylation signal and the C- terminal regulatory tyrosine. TXK expression is detected primarily in T cells and some myeloid cell lines but not in a number of other cell types. TXK shares 60% amino acid homology with EMT and 57% with BTK ov er the SH3, SH2 (Src-homology) and catalytic domains but unlike BTK, E MT and tee, it lacks Gap 1 homology and steroid hormone receptor homol ogy in the N-terminal region. Genomic clones containing TXK have been isolated and hybridize to chromosome position 4p12.