N. Jausonsloffreda et al., CHIMERIC RECEPTORS AS A TOOL FOR LUMINESCENT MEASUREMENT OF BIOLOGICAL-ACTIVITIES OF STEROID-HORMONES, Journal of bioluminescence and chemiluminescence, 9(3), 1994, pp. 217-221
Some applications of chimeric cellular models are presented to study t
he biological activities of steroid hormones. We have used several chi
meric constructs encoding the DNA binding domain of Gal4 yeast protein
fused to the hormone binding domain of various steroid receptors (M R
, PR, G R and ER). Interactions of these chimeric receptors with a 17-
mer DNA sequence. specific for Gal-4, control expression of the firefl
y luciferase as a reporter gene. Stable transfected cell lines express
ing the firefly luciferase under the control of different steroids wer
e established and an efficient and easy sub-cloning was allowed with t
he help of an imaging system using a single-photon-counting camera. In
the cell lines obtained, the bioluminescent response can be easily me
asured and thus used to measure specific biological activities of ster
oid agonists or antagonists. We observed that the responses are effect
or-concentration-dependent and their biological activities will be com
pared to those of native receptors.