CHIMERIC RECEPTORS AS A TOOL FOR LUMINESCENT MEASUREMENT OF BIOLOGICAL-ACTIVITIES OF STEROID-HORMONES

Citation
N. Jausonsloffreda et al., CHIMERIC RECEPTORS AS A TOOL FOR LUMINESCENT MEASUREMENT OF BIOLOGICAL-ACTIVITIES OF STEROID-HORMONES, Journal of bioluminescence and chemiluminescence, 9(3), 1994, pp. 217-221
Citations number
19
Categorie Soggetti
Biology
ISSN journal
08843996
Volume
9
Issue
3
Year of publication
1994
Pages
217 - 221
Database
ISI
SICI code
0884-3996(1994)9:3<217:CRAATF>2.0.ZU;2-X
Abstract
Some applications of chimeric cellular models are presented to study t he biological activities of steroid hormones. We have used several chi meric constructs encoding the DNA binding domain of Gal4 yeast protein fused to the hormone binding domain of various steroid receptors (M R , PR, G R and ER). Interactions of these chimeric receptors with a 17- mer DNA sequence. specific for Gal-4, control expression of the firefl y luciferase as a reporter gene. Stable transfected cell lines express ing the firefly luciferase under the control of different steroids wer e established and an efficient and easy sub-cloning was allowed with t he help of an imaging system using a single-photon-counting camera. In the cell lines obtained, the bioluminescent response can be easily me asured and thus used to measure specific biological activities of ster oid agonists or antagonists. We observed that the responses are effect or-concentration-dependent and their biological activities will be com pared to those of native receptors.