CHROMOSOME LOCALIZATIONS OF GENES FOR 5 CAMP-SPECIFIC PHOSPHODIESTERASES IN MAN AND MOUSE

Cyclic nucleotides are important second messengers that mediate a numb er of cellular responses to external signals. Cyclic nucleotide phosph odiesterases play a role in signal transduction by regulating the cell ular concentrations of these messengers. Here, we have applied Souther n analyses of somatic cell hybrid lines and of recombinant inbred (RI) mouse strains as well as fluorescence chromosomal in situ hybridizati on (FISH) to chromosomally localize Jive cAMP-specific nucleotide phos phodiesterase genes in human and mouse. Genes DPDE1, DPDE2, DPDE3, and DPDE4 that share sequence homology with the Drosophila dunce gene wer e assigned to human chromosomes 19 (DPDE1 and DPDE2) 5q12 (DPDE3), and 1p31 (DPDE4) and to mouse chromosomes 8, 9, 13, and 4, respectively. The high-affinity cAMP-specific phosphodiesterase gene (HCP1) was mapp ed to human chromosome 8q13-q22. Since these genes are potential candi dates for involvement in psychiatric or behavioral disorders, knowledg e of their chromosomal localizations will facilitate the discovery of their association with disease genes as they ave being mapped by linka ge studies.