PHYSICAL MAP OF SMALL-CELL LUNG-CANCER DELETION REGION ON SHORT ARM OF HUMAN-CHROMOSOME-3 (3P13-22) BASED ON RADIATION FUSION HYBRID ANALYSIS

Deletion of DNA sequences from various regions of the shell arm of hum an chromosome 3 (3p13-14, 3p21, and 3p25) has been observed during the development of a variety of solid tumors, including lung and renal ce ll carcinomas. In this study we have used a set of radiation fusion hy brids to generate a physical map of chromosome 3p to orient the search for putative tumor suppressor genes. Eighty-six human-hamster radiati on fusion hybrids were screened on Southern blots for the retention of 55 human chromosome 3p DNA markers. The high marker density enabled u s to identify a set of successively overlapping chromosome fragments i n the 3p13-22 area guided by eight markers with previously known order . Twenty-four map intervals were e suggested using breakpoints determi ned by partial fragment overlaps. The final order between the markers derived is consistent with previous information about localizations fo r 26 of the markers to three larger cytogenetic intervals.