EFFECT OF GLUCOSE ON LIPID-PEROXIDATION AND ANTIOXIDANT ENZYME-ACTIVITIES IN RAT-LIVER SLICES

Glucose induced lipid peroxidation in rat liver slices and caused memb rane damage to liver cells due to oxidative stress. Superoxide dismuta se provided an antioxidant enzyme protection against the oxidative str ess at low glucose concentrations (25-75 mM) but catalase showed no si gnificant changes. The activity of both of these enzymes decreased in the liver slices at a higher concentration of glucose (150 mM). The gl utathione content and the activity of glutathione S-transferase in the liver slices was decreased in the presence of glucose. The decrease i n the glutathione content and glutathione S-transferase activity cause ineffective neutralization of the electrophiles. These findings indic ate that glucose induces lipid peroxidation and alters the activity of superoxide dismutase and catalase in the liver slices. The decreased glutathione content and glutathione S-transferase activity may be the reason for increased lipid peroxides.