CRYPT CELL THYMIDINE INCORPORATION IN ILEAL URINARY POUCH - PRELIMINARY-REPORT

This is a brief communication about mucosal change in ileal urinary re servoirs, the earliest reported observations to date. Previous authors described early villous atrophy and crypt elongation, late panmucosal atrophy and metaplasia. Crypt cell proliferation should be a forerunn er of these events and therefore warrants quantitative measurement. We studied continent pouches in dogs on postoperative days 42 and 43 usi ng in vivo tritiated thymidine incorporation into crypt cell DNA, a no vel application of autoradiography for urinary reservoirs. Adjacent no rmal ileum provided the internal control. Increased crypt cell thymidi ne incorporation was confirmed at this early time.