Py. Rescan et al., IDENTIFICATION OF A MUSCLE FACTOR-RELATED TO MYOD IN A FISH SPECIES, Biochimica et biophysica acta, N. Gene structure and expression, 1218(2), 1994, pp. 202-204
We have isolated the cDNA encoding a myogenic factor expressed in embr
yonic trout muscle by hybridization with a Xenopus MyoD cDNA. Nucleoti
de sequence analysis and amino acid comparison showed that this cDNA c
alled TMyoD encodes a polypeptide of 276 amino acids with 70% identity
to the entire Xenopus MyoD protein and 92% identity within the basic
and myc-like region. Results from Northern blotting showed that the co
rresponding transcript is expressed both in adult and embryonic skelet
al musculature and in an in vitro myogenesis system, but is undetectab
le in cardiac and smooth muscles and in non muscle tissues.