A critical review of the literature suggests that the microenvironment
of dentinal tubules appears to favour the selection of relatively few
bacterial types irrespective or the aetiology of the infection proces
s; coronal dental caries or pulpar necrosis. These bacteria may consti
tute an important reservoir from which root canal infection and reinfe
ction may occur following pulp necrosis or during and after endodontic
treatment. Previous studies of this microflora have utilized microbio
logical culture techniques which need to be supplemented by those that
allow in situ demonstration as well as identification of the bacteria
. Newer treatment strategies that are designed to eliminate this micro
flora must include agents that can penetrate the dentinal tubules and
destroy these microorganisms, since they are located in an area beyond
the host defence mechanisms where they cannot be reached by systemica
lly administered antimicrobial agents.