YEAST SERINE ISOACCEPTOR TRANSFER-RNAS - VARIATIONS OF THEIR CONTENT AS A FUNCTION OF GROWTH-CONDITIONS AND PRIMARY STRUCTURE OF THE MINOR TRNA(SER)GCU

Authors
HEYMAN T AGOUTIN B FIX C DIRHEIMER G KEITH G
Citation
T. Heyman et al., YEAST SERINE ISOACCEPTOR TRANSFER-RNAS - VARIATIONS OF THEIR CONTENT AS A FUNCTION OF GROWTH-CONDITIONS AND PRIMARY STRUCTURE OF THE MINOR TRNA(SER)GCU, FEBS letters, 347(2-3), 1994, pp. 143-146
Citations number
11
Categorie Soggetti
Biophysics,Biology
Journal title
FEBS letters → ACNP
ISSN journal
00145793
Volume
347
Issue
2-3
Year of publication
1994
Pages
143 - 146
Database
ISI
SICI code
0014-5793(1994)347:2-3<143:YSIT-V>2.0.ZU;2-P
Abstract
The primary structure of Saccharomyces cerevisiae tRNA(Sec)GCU is pres ented (EMBL database accession No. X74268 S. cerevisiae tRNA-Ser). In addition, quantitation of the relative amounts of serine isoaccepting tRNAs in yeast grown on different media showed that the minor tRNA(Sec )GCU decreased while the major tRNA(Sec)AGA increased as the growth ra te and the cellular protein content increased. The minor species, tRNA (Sec)CGA and tRNA(Sec)UGA, were not separated by our gel system, howev er, taken together they appeared to vary in the same way as tRNA(Sec)G CU. These data suggest a growth rate dependence of yeast tRNAs similar to that previously described for F coli tRNAs.