SPECTROFLUOROMETRIC METHOD FOR NO DETERMINATION

Authors
GABOR G ALLON N
Citation
G. Gabor et N. Allon, SPECTROFLUOROMETRIC METHOD FOR NO DETERMINATION, Analytical biochemistry, 220(1), 1994, pp. 16-19
Citations number
24
Categorie Soggetti
Biology
Journal title
Analytical biochemistry → ACNP
ISSN journal
00032697
Volume
220
Issue
1
Year of publication
1994
Pages
16 - 19
Database
ISI
SICI code
0003-2697(1994)220:1<16:SMFND>2.0.ZU;2-O
Abstract
A spectroscopic method for the quantitative determination of NO in aqu eous solution is presented. The method is based on the uv absorbance o f nitrosothiols, coupled with the inner filter effect. A threshold of 5.10(-7) M with a wide dynamic range of 5.10(-7)-10(-4) M are obtained . This method enables measurements in brain homogenates. Ultimately, s ince this method is immune to electrical perturbances, it will be appl ied at the tip of an optical fiber for in situ monitoring of NO or glu tathione in brain and blood vessels. (C) 1994 Academic Press,Inc.