MONOCLONAL-ANTIBODIES AGAINST HUMAN TETRANECTIN, EPITOPE CHARACTERIZATION AND USE IN IMMUNOHISTOCHEMISTRY

Two IgG2a/kappa- and three IgG1/kappa monoclonal antibodies (MAbs) wer e produced against human tetranectin (TN): this is the first report of stable hybridomas producing MAbs against TN. All the MAbs reacted wit h non-conformational epitopes located within amino acid residues 50-18 1 of the primary sequence. In relative epitope mapping with enzyme imm unoassay and isotachophoresis the five MAbs defined two independent ep itope groups. One of them is suggested to be immunodominant in rabbits , since MAbs inhibited binding of a polyclonal rabbit antibody. Severa l combinations of MAbs were suitable for TN-ELISA and two MAbs could b e used for immunohistochemical detection of TN in both fresh frozen an d paraffin-embedded tissues. The MAbs will facilitate future studies o n structure, function, clinical significance and immunolocalization of TN. (C) 1997 Elsevier Science B.V.