EFFECT OF LOOP DIURETICS ON RAT PERITONEAL AND HUMAN LUNG MAST-CELLS

The effects of furosemide and bumetanide on immunologically stimulated rat peritoneal and human lung mast cells were compared. Furosemide an d bumetanide had different modulatory actions on the rat peritoneal ma st cell. Furosemide inhibited anti-IgE-induced histamine release. Prei ncubation of the cells with the drug, prior to anti-IgE stimulation, s ignificantly reduced furosemide's inhibitory effect. In contrast, bume tanide potentiated anti-IgE-induced histamine secretion from the rat p eritoneal mast cell. Both diuretics were modest inhibitors of anti-IgE -mediated histamine release from human lung mast cells. For furosemide , inhibition decreased with preincubation, while preincubation increas ed bumetanide's inhibitory action.