IMMUNOHISTOCHEMICAL STUDIES WITH SKIN MAST-CELLS

Using different histochemical and immunohistochemical methods we demon strate that isolated skin mast cells used for different pharmacologica l and biochemical studies exhibit the same staining pattern compared t o skin mast cells in situ. Surface structures and enzyme content of th e cells appear not to be influenced by the isolation technique. We als o observed no differences in the staining pattern of mast cells in neu rofibroma, a cutaneous disorder characterized by an increase of mast c ell numbers.