EVIDENCE FOR AN ALTERED T-CELL RECEPTOR REPERTOIRE IN CROHNS-DISEASE

We have compared the frequencies of T cells expressing each of four di fferent T cell receptor (TCR) V beta segements in lamina propria and p eripheral blood lymphocytes of 12 Crohn's disease (CD), six ulcerative colitis (UC), and 10 control patients in an attempt to identify disea se-specific changes. The frequencies of CD4+ and CD8+ cells reacting w ith each of four fluoresceinated TCR-specific monoclonal antibodies di rected against V beta 5, V beta 6.7a, V beta 8, and V beta 12 were det ermined by now cytometry. There was no difference among the groups in the average frequency of any single V beta segment in either the CD4or CD8+ subpopulations. However, when the sum of the differences in V beta frequencies (Delta score) between peripheral blood lymphocytes (P BL) and lamina propria lymphocytes (LPL) were determined for each indi vidual, significant differences were observed between the CD4+ and CD8 + populations and among the patient groups. In all three patient group s, there were significant individual differences between LPL and PBL i n the frequencies of CD8+ and CD4+ cells reacting with the four V beta -specific mAb. In Controls and UC, this difference was, on average, tw o-fold greater in CD8+ cells than in CD4+. In CD, however, this differ ence was, on average, the same for CD8+ and CD4+ cells. These observat ions suggest that (1) the human colonic LPL TCR repertoire is normally different from that of PBL, especially in the CD8+ population and (2) there is an alteration in the LPL TCR repertoire in CD which is not o bserved in Controls or UC.