This study examined the antibacterial activities of several commercial
ly available glass ionomer cements, dentin bonding systems and luting
agents by employing both agar plate diffusion (APD) and growth inhibit
ion (GI) methods. Amalgam and resin composites were also tested as con
trol materials. In both methods (APD and GI), cylindrical specimens we
re used. Four bacteria strains were tested: Streptococcus mutans, S. s
alivarius, S. mitis and S. sanguis. These studies were performed using
standardized innoculums with selective media, and the assayed materia
ls were directly applied on the assay cultures and plates. The results
of agar plate assay were in accordance with the results of growth inh
ibition method. The glass ionomer cements showed marked antibacterial
activity. On the contrary, amalgam, composites, luting agents and dent
inal bonding systems did not affect bacterial growth. The sensitivity
of the growth method showed that all the strains were inhibited in the
same way by each inhibitory material. The data suggest that the use o
f glass ionomer cements as cavity liners/bases may reduce the conseque
nces of microleakage due to its antibacterial properties.