EXPRESSION OF INDUCIBLE NITRIC-OXIDE SYNTHASE BY NEURONS FOLLOWING EXPOSURE TO ENDOTOXIN AND CYTOKINE

In the CNS, nitric oxide (NO) has been implicated as both a mediator o f neurotoxicity and a neuromodulator. The inducible NO synthase (iNOS) , thought to mediate toxic effects of NO, has been attributed to glial cells in the CNS. We now report that cerebellar granule cell neurones can be stimulated by lipopolysaccharide and interferon-gamma to expre ss iNOS in vitro, as demonstrated by reverse transcription-polymerase chain reaction and fluorescent in situ hybridisation. The expression o f both constitutive NO synthase (cNOS) and iNOS by neurones suggests t hat NO has diverse functions in the brain, and supports the possibilit y that iNOS plays a role in neuronal damage and inflammation following activation of brain microglia and production of cytokines.