INDEPENDENT EXPRESSION OF THE FIRST 2 TRIPLE GENE BLOCK PROTEINS OF BEET NECROTIC YELLOW VEIN VIRUS COMPLEMENTS VIRUS DEFECTIVE IN THE CORRESPONDING GENE BUT EXPRESSION OF THE 3RD PROTEIN INHIBITS VIRAL CELL-TO-CELL MOVEMENT
C. Bleykastengrosshans et al., INDEPENDENT EXPRESSION OF THE FIRST 2 TRIPLE GENE BLOCK PROTEINS OF BEET NECROTIC YELLOW VEIN VIRUS COMPLEMENTS VIRUS DEFECTIVE IN THE CORRESPONDING GENE BUT EXPRESSION OF THE 3RD PROTEIN INHIBITS VIRAL CELL-TO-CELL MOVEMENT, Molecular plant-microbe interactions, 10(2), 1997, pp. 240-246
Cell-to-cell movement of beet necrotic yellow vein furovirus is contro
lled by three slightly overlapping genes on RNA 2 called the triple ge
ne block (TGB) encoding, in order, P42, P13, and P15, Synthesis of P42
is directed by subgenomic RNA 2sub(a) while synthesis of both P13 and
P15 is probably directed by a dicistronic subgenomic RNA, 2sub,, For
complementation experiments, each TGB protein gene was inserted into a
''replicon'' derived from viral RNA 3, In mixed infections, the repli
cons expressing P42 and P13 complemented RNA 2 mutants defective in th
e corresponding gene, A P15-containing replicon did not complement a P
15-defective RNA 2 but complementation was observed with a dicistronic
replicon containing the P15 gene placed behind the P13 gene. In mixed
infections with wild-type viral RNAs, the P15-containing replicon did
not inhibit viral RNA replication in protoplasts but blocked local le
sion formation on leaves,Infection of leaves was also inhibited by an
RNA3-derived replicon containing the third TGB gene from another furov
irus, peanut clump virus. The results are consistent with a model in w
hich viral cell-to-cell movement requires production of appropriate re
lative amounts of P13 and P15, and their expression from a dicistronic
subgenomic RNA provides a mechanism for coordinating their synthesis.