STABILITY AND STORAGE-CONDITIONS OF NADH-CYTOCHROME B5 REDUCTASE CROSS-LINKED INTO GELATIN BY CHROMIUM(III) ACETATE

Authors
YILDIRIM O AKBULUT U ARINC E SUNGUR S
Citation
O. Yildirim et al., STABILITY AND STORAGE-CONDITIONS OF NADH-CYTOCHROME B5 REDUCTASE CROSS-LINKED INTO GELATIN BY CHROMIUM(III) ACETATE, Biomaterials, 15(8), 1994, pp. 587-592
Citations number
24
Categorie Soggetti
Engineering, Biomedical","Materials Science, Biomaterials
Journal title
Biomaterials → ACNP
ISSN journal
01429612
Volume
15
Issue
8
Year of publication
1994
Pages
587 - 592
Database
ISI
SICI code
0142-9612(1994)15:8<587:SASONB>2.0.ZU;2-W
Abstract
NADH-cytochrome b5 reductase was isolated and partially purified from rabbit liver microsomes. It was immobilized into gelatin by chemical c ross-linking. Chromium (III) acetate was used as cross-linker. The eff ects of pH and temperature on the immobilized cytochrome b5 reductase were investigated. The reusability and storage stability of immobilize d enzyme were also tested. Immobilized NADH-cytochrome b5 reductase ac tivities were found to be stable for at least 72 d and 24 uses. The st orage stability of NADH-cytochrome b5 reductase was improved with immo bilization at 25 degrees C.