SYNTHETIC PEPTIDES FROM THE REGION 30-54 OF THE INTERLEUKIN-2 RECEPTOR BETA-CHAIN (IL-2R-BETA) AFFECT IL-2 BINDING TO HUMAN T-CELLS

Authors
DRAKOPOULOU E SOTIROPOULOU G TSILIVAKOS V GEORGOULIAS V CORDOPATIS P
Citation
E. Drakopoulou et al., SYNTHETIC PEPTIDES FROM THE REGION 30-54 OF THE INTERLEUKIN-2 RECEPTOR BETA-CHAIN (IL-2R-BETA) AFFECT IL-2 BINDING TO HUMAN T-CELLS, Collection of Czechoslovak Chemical Communications, 59(4), 1994, pp. 943-950
Citations number
26
Categorie Soggetti
Chemistry
Journal title
Collection of Czechoslovak Chemical Communications → ACNP
ISSN journal
00100765
Volume
59
Issue
4
Year of publication
1994
Pages
943 - 950
Database
ISI
SICI code
0010-0765(1994)59:4<943:SPFTR3>2.0.ZU;2-P
Abstract
The interleukin-2 receptor beta-chain (IL-2Rbeta) is responsible for t he IL-2 internalization and signal transduction. To identify domains f rom the IL-2Rbeta extracellular region which are involved in ligand-re ceptor interactions, eight peptides of 8 to 25 amino acids correspondi ng to IL-2Rbeta sequence 30-54 were synthesized. Their biological effe ct was tested with [I-125]IL-2 equilibrium binding to PHA-blasts. Prei ncubation of [I-125]IL-2 with peptides IL-2Rbeta (35-54) and (30-42) a ffects IL-2 binding to both high and intermediate affinity receptors b y revealing a greater number of active sites with lower affinity.