ASSIGNMENT OF GENES TO PULSE-FIELD SEPARATED CHROMOSOMES OF SCHIZOPHYLLUM-COMMUNE

Chromosomal DNAs of the basidiomycete Schizophyllum commune were separ ated by Contour-Clamped Homogeneous Electric Field Electrophoresis (CH EF). The estimated sizes of the chromosomal DNAs ranged from 4.7 Megab ase pairs (Mbp) to 1.6 Mbp, totalling 35.6 Mbp. Using sequences from 2 0 cloned genes we obtained hybridization signals to at least 8 out of 11 chromosomal DNAs. The mating-type genes A and B were mapped to the largest and the smallest chromosomal DNA band, respectively. Developme ntally regulated genes which are under the control of the mating-type genes, were localized to five different chromosomal DNA bands.