HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC SCREENING METHODS FOR REVEALING THE STEREOSELECTIVITY OF REVERSIBLE INTERACTIONS BETWEEN LIGAND ENANTIOMERS AND A COUNTERPART SUBSTANCE

The stereoselectivity of the reversible binding interaction between tr yptophan enantiomers and various counterpart substances (oligo- and po lysaccharides, proteins) has been studied by HPLC. The properties of t he ,,simplest'' of three different experimental designs are presented. Bovine serum albumin bound to silica gel was used as the chiral HPLC stationary phase; phosphate-buffered DL-tryptophan solution was used a s mobile phase. The stereoselectivity of interactions between differen t oligo- and macrobiomolecules and the two tryptophan enantiomers was evaluated from the areas of negative recorder deflections at the reten tion times of the D and L isomers of tryptophan.