APPLICATION OF LIQUID-CHROMATOGRAPHY PARTICLE-BEAM MASS-SPECTROMETRY AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY FOR THE IDENTIFICATION OF METABOLITES OF POLYCYCLIC AROMATIC-HYDROCARBONS
Tj. Gremm et Fh. Frimmel, APPLICATION OF LIQUID-CHROMATOGRAPHY PARTICLE-BEAM MASS-SPECTROMETRY AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY FOR THE IDENTIFICATION OF METABOLITES OF POLYCYCLIC AROMATIC-HYDROCARBONS, Chromatographia, 38(11-12), 1994, pp. 781-788
Two liquid chromatography-particle beam mass spectrometry (LC-PBMS) sy
stems have been used for analysis of polycyclic aromatic hydrocarbon m
etabolites in water samples from a biological decomposition model expe
riment. The results were compared with those obtained by classical gas
chromatography-mass spectrometry. Evaluation of spectral data indicat
ed the presence of six main metabolites; 1H,3H-naphtho [1,8-cd]pyran-1
,3-dione, 1-(hydroxymethyl)naphthalene, and 1,2-acenaphthenedione were
identified by use of standard substances and spiking experiments. Enr
ichment of the investigated water samples with online pre-column (C-18
) Concentration, freeze-drying, and solvent extraction led to similar
results with only slight differences. The application of microbore sep
aration columns proved to be a promising tool in particle beam LC-MS m
easurements.