PAB1614, A MONOCLONAL-ANTIBODY REACTIVE WITH THE TUMOR-ANTIGENS OF SV40, JC, BK, AND POLYOMA-VIRUS, AND OTHER JC VIRUS TUMOR-ANTIGEN CROSS-REACTIVE ANTIBODIES OF THE PAB1601-1636 PANEL

PAb1614, an SV40-specific monoclonal antibody of the panel PAb1601-163 6 reacts with large and small tumor antigens of SV40, BK and JC virus, and with polyoma virus large and middle tumor antigens, but not with the large tumor antigen of the lymphotropic papova virus. Using immuno fluorescence and immunoblot competition assays and ELISA with syntheti c peptides, it is shown that the epitope is represented by the SV40 tu mor antigen undecapeptide, K39-E49. This peptide comprises the tumor a ntigen consensus sequence, H42-G47, of the polyoma viruses. However, t he epitope of PAb1614 probably does not exactly coincide with this hex apeptide. This explains why some cross-reactions are less strong, or a bsent, as in the case of the lymphotropic papova virus. Further antibo dies of the PAb1601-1636 panel that cross-react with the JC virus larg e tumor antigen are PAb1602, 1604, 1606, 1618, 1621, 1622, 1623, 1624, 1626, 1629, and 1633.