FLOW-INJECTION CHEMILUMINESCENCE DETERMINATION OF THE HYDRAZONES OF AROMATIC KETONES

A flow-injection system for investigating the chemiluminescence of aro matic ketone hydrazones is developed, based on KMnO4 oxidation with fo rmic acid as a carrier. Rhodamine B has proved to be an efficient sens itizer. The hydrazones studied are benzophenone hydrazone, benzil dihy drazone, acetophenone hydrazone and benzoin hydrazone. The relative st andard deviation for ten replicate injections of 1 x 10(-5) M benzophe none hydrazone and the detection limit (3 sigma) are 2% and 3 x 10(-6) M (30 pmol, 10-mu l injection), respectively. The interference effect of some metal ions is also investigated. Cu(II), Cd(II), Zn(II), Ca(I I), Co(II) and Ni(II) have no effect whilst Mn(II), Mo(VI) and Ce(IV) suppress the signal.