Tracking of animals mark with fluorescent pigment has been used primar
ily in studies of habitat use and home range, without considering time
as a factor. We determined the length of time that fluorescent pigmen
t remains effective in marking trails of Eastern Chipmunks (Tamias str
iatus) in Itasca State Park, Minnesota. Chipmunks were released and re
captured at specific times during the same day. We found that fluoresc
ent pigments were effective in marking trails of chipmunks for a mean
an time of 6.61 hours with a maximum time of 7.75 hours. The amount of
grooming the chipmunks performed was the most important factor influe
ncing the duration the fluorescent pigment effectively leaves a trail.