Sg. Chang et al., INTERLEUKIN-6 PRODUCTION IN PRIMARY HISTOCULTURE BY NORMAL HUMAN KIDNEY AND RENAL TUMOR-TISSUES, Anticancer research, 17(1A), 1997, pp. 113-115
Interleukin-6 (IL-6) is a multifunctional cytokine with many biologic
activities in vitro, including synergistic or antagonistic actions wit
h one or more other cytokines. The role and induction parameters of IL
-6 in renal cell carcinoma (RCC) are not fully understood. To understa
nd the ability of RCC to produce IL-6, we determined the IL-6 concentr
ation in the supernatant of histocultured human normal kidney, RCC, re
nal Wilms' tumor and renal oncocytoma. From these studies, we conclude
that the kidney is one of the main sources of IL-6. Normal renal cort
ical tissues and renal tumors can produce IL-6 in histoculture without
stimulation. Thus histoculture supports the long-term production of I
L-6, potentially allowing many important studies of this cytokine in t
he normal and malignant kidney.