DETECTION AND ANALYSIS OF HUMAN PAPILLOMAVIRUS-16 AND PAPILLOMAVIRUS-18 HOMOLOGOUS DNA-SEQUENCES IN ORAL LESIONS

The prevalence of human papillomavirus (HPV) 16 and 18 was investigate d in oral lesions of the population of northeast China including squam ous cell carcinomas (SCCs), candida leukoplakias, lichen planuses and pallillomas, by southern blot hybridization with polymerase chain reac tion (PCR). Amplified HPV16 and 18 E6 DNA was analyzed by cycle sequen ce. HPV DNA was detected in 14 of 45 SCCs (31.1%). HPV18 E6 DNA and HP V16 E6 DNA were detected in 24.4% and 20.0% of SCCs, respectively. Dua l infection of both HPV 16 and HPV 18 was detected in 6 of 45 SCCs (13 .3%), but not in other oral lesions. HPV 18 E6 DNA was also detected i n 2 of 3 oral candida leukoplakias, but in none of the 5 papillomas. O ur study indicated that HPV 18 infection might be more frequent than H PV 16 infection in oral SCCs in northeast Chinese, dual infection of h igh risk HPV types was restricted in oral SCCs, and that HPV infection might be involved in the pathogenesis of oral candida leukoplakia.