The recent developpement of co-culture technique has permited to cryop
reserve human embryos at the blastocyst stage. The aim of the present
study was to compare pregnancy rates after transfers of embryos frozen
at an early embryo stage (2-8 cell stage, group A; n = 175) with othe
rs frozen at blastocyst stage (group B; n = 98). Our results demonstra
ted that the frozen / thawed blastocyst survival is aproximately 100 %
. In comparison the early embryos survival rate was 59 % (P < 0,05). M
oreover, pregnancy rates / embryo transfer in group A (16,4 %) were si
gnificantly improved by comparison to those observed with early embryo
s (6,2 %) (P < 0,05). In conclusion, our results demonstrate that embr
yo frozen / thawed at the blastocyst stage can be useful for improving
pregnancy rates after cryopreservation.