ANTIBODIES SPECIFIC FOR THE NEURONAL FORM OF THE SRC PROTEIN ELICITEDBY AN ANTIGENIZED ANTIBODY

To elicit antibodies directed specifically against the neuron-specific form of the c-sre gene product, pp(60c-src(+)), we used an antigenize d antibody comprising a decamer containing the amino acid sequence spe cific to pp(60c-src(+)) inserted into the third hypervariable loop of the heavy (H)-chain variable (V)-region. This was used to raise anti-i diotype antibodies reacting with the peptide epitope in rabbits. The a ntisera reacted with pp(60c-src(+)), as judged by immune blotting, imm unoprecipitation, immune complex kinase assay, and indirect immunofluo rescence staining, but did not react with the fibroblast form of the c -sre gene product, pp(60c-scr). Antigenized antibody is a useful appro ach for producing antibodies able to distinguish between isoforms of t he same gene product and specific for the neuronal form of the Src pro tein.