Supernatants derived from desiccated plant material gathered from Agro
stis/Festuca vegetation had an inhibitory effect on the germination an
d early development of seedlings of Trifolium repens cv. S184. Two com
pounds, o-hydroxyphenylpropionic acid and benzoic acid, were identifie
d chromatographically and their structures confirmed by mass spectrome
try. Commercial preparations of the two compounds were effective inhib
itors at a concentration of 10(-2) and 10(-3) M respectively when T re
pens was used as the phytometer species. Benzoic acid lost its inhibit
ory effect when the pH of the two solutions was adjusted to pH 5.5. Th
e roots of seedlings of white clover were severely distorted by o-hydr
oxyphenylpropionic acid at 10(-3) M. It is likely that phenolic acids,
produced from the surface trash created by slot seeding procedures, i
nterfere with the establishment of white clover in upland pastures.