ENHANCED CHIRAL SEPARATION OF DANSYLATED AMINO-ACIDS WITH CYCLODEXTRIN DEXTRAN POLYMER NETWORK BY CAPILLARY ELECTROPHORESIS

A method for the chiral separation of dansyl-DL-amino acid mixtures us ing a dextran (M(r) 2 000 000) polymer network containing beta-cyclode xtrin (beta-CD) in HPCE was developed. Mixtures of up to seven amino a cids could be baseline resolved by this method under neutral pH condit ions. Resolution was dependent on the dextran concentration in the pol ymer network. Temperature effects on the chiral separation were studie d. Optimal efficiency and resolution of DL-enantiomeric pairs of amino acid samples were obtained at 25 degrees C. The resolution of differe nt amino acids used in this work decreased with an increase in tempera ture.