Mm. Rogan et al., ENANTIOMERIC SEPARATION OF SALBUTAMOL AND RELATED IMPURITIES USING CAPILLARY ELECTROPHORESIS, Electrophoresis, 15(6), 1994, pp. 808-817
The enantiomeric resolution of salbutamol and its chiral and achiral r
elated impurites is investigated using capillary electrophoresis. The
effects of nine varieties of cyclodextrin, cyclodextrin concentration
and organic modifier concentration were studied in an attempt to resol
ve all possible analytes in a complex mixture of salbutamol-related so
lutes. Eleven components including three enantiomeric pairs were basel
ine resolved using 112 mM dimethyl-beta-cyclodextrin at pH 2.5 in a ci
tric acid/phosphate buffer. Both methanol and iso-propyl alcohol at up
to 20% were found to have a deleterious effect on the separation. Bin
ding constants and mobility values for the free and complexed forms fo
r each solute were determined. The results are interpreted by consider
ing the physical properties of the molecules under the conditions empl
oyed and a rationale proposed for the underlying basis for chiral and
achiral selectivity.