QUANTITATIVE APPLICATIONS AND VALIDATION OF THE RESOLUTION OF ENANTIOMERS BY CAPILLARY ELECTROPHORESIS

The separation and quantitation of enantiomers is an important applica tion of capillary electrophoresis. This paper reviews the performance and validation of capillary electrophoretic methods for this applicati on. Limits of detection of < 0.1% are shown for determinations of mino r enantiomer levels. Application areas include enantiomeric purity tes ting of pharmaceuticals and herbicides, reaction rate monitoring, stab ility testing, and clinical analysis. It is concluded that the number of quantitative application areas will undoubtedly expand as capillary electrophoresis becomes more widely established as an alternative and complement to high-performance liquid chromatography.