CYTOMEGALOVIRUS AND CERVICAL-CANCER - FAILURE TO DETECT A DIRECT ASSOCIATION OR AN INTERACTION WITH HUMAN PAPILLOMAVIRUSES

To investigate the possibility of human cytomegalovirus (HCMV) involve ment in the aetiology of cervical carcinoma or in the development of a more clinically aggressive cancer cell phenotype, biopsies of cervica l cancer from 103 women undergoing primary therapy for invasive (Stage Ia to IV) cervical cancer were investigated using a polymerase chain reaction (PCR) designed to detect sequences from the IE mtrII region o f HCMV. PCR assays were also used on the same specimens to identify th e presence of common human papillomavirus (HPV) types associated with cervical cancer (HPV 16, 18, 31, 33, 52, and 58). Of the 103 cancers e xamined, only 4 contained detectable HCMV DNAs, a proportion lower tha n that found by another Australian group investigating the cervical ca rriage of HCMV in women with normal cervices. In contrast, 89 of these cancers were positive for HPV DNA sequences, with HPV 16 (65/103) and HPV 18 (17/103) being most commonly detected. Three of the 4 HCMV-pos itive tumors were also positive for HPV 16 DNA. An examination of the relevant histopathological and clinical data revealed no evidence to s upport a contention that cancers positive for HCMV are associated with any unusual histologic cell types or with aggressive clinical behavio r. (C) 1994 Academic Press, Inc.