Gy. Liu et al., PREPARATION OF MILLIGRAMS OF PURE ENANTIOMERS USING ANALYTICAL-SCALE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Chirality, 6(4), 1994, pp. 290-294
Pure enantiomers of an agrochemical process intermediate, ,4-dimethyl-
2-(1H-1,2,4-triazol-1-yl)-pentan-3-one (1), have been prepared on the
milligram scale under overload chromatographic conditions on an analyt
ical chiral column (250 x 4.6 mm i.d.). The effects of variation of te
mperature and mobile phase composition on retention factor, separation
factor, and peak resolution have been investigated. Effects,of now ra
te, enantiomer ratio, sample concentration, and column load on product
ivity are also studied. Seven milligrams of the less retained (+)-enan
tiomer and 5 mg of the (-)-enantiomer were obtained from a single inje
ction of 21 mg of (RS)-1. (C) 1994 Wiley-Liss, Inc.