THIN-LAYER AND HIGH-PRESSURE LIQUID-CHROMATOGRAPHIC DETERMINATION OF HISTAMINE IN FISH-TISSUES

TLC and HPLC techniques have been examined for histamine determination in fish. In case of HPLC determination a comparison of the performanc e characteristics of UV-Vis and Fluorescence detection is also describ ed. Regression analysis was performed between the two sets of data, in order to examine the relative efficiency of the two techniques for th e determination of histamine in fish sample extracts. Either of the tw o techniques proved sufficient for the determination of histamine in c ommon concentration ranges in fish extracts although fluorescence dete ction had better sensitivity. The recovery of histamine from fish samp les was decreased in comparison to the standard solutions but it was s till sufficient for routine analysis of the fish samples.