IN-VIVO MODULATION OF RAT DISTAL TUBULE NET HCO3 FLUX BY VIP, ISOPROTERENOL, ANGIOTENSIN-II, AND ADH

To examine the in vivo effects of agonists reported to influence bicar bonate flux (J(tCO2)), microperfusion experiments were carried out on distal tubules of normally fed or overnight-fasted rats. As we previou sly reported, distal tubules from fed rats reabsorbed no bicarbonate, whereas overnight-fasted rats consistently reabsorbed, bicarbonate (J( tCO2) 10 +/- 3 pmol.min(-1).mm(-1); P < 0.01). Vasoactive intestinal p eptide and isoproterenol infused intravenously (7.3 and 4.0 mu g.kg(-1 ).h(-1), respectively) in fasted rats suppressed J(tCO2) and, in the c ase of vasoactive intestinal peptide, elicited net bicarbonate secreti on (J(tCO2) - 10 +/- 2 and -4 +/- 4 pmol.min(-1).mm(-1), respectively) . In fed rats, angioten sin II infused at a rate of 1.2 mu g.kg(-1).h( -1) stimulated bicarbonate reabsorption (J(tCO2) 16 +/- 3 pmol.min(-1) .mm(-1)), while antidiuretic hormone infused at 0.024 mu g.kg(-1).h(-1 ) elicited a similar response (17 +/- 4 pmol.min(-1).mm(-1)), both val ues being significantly different from control. These results, therefo re, demonstrate for the first time that these agonists can modulate J( tCO2) at the distal tubule site in vivo and therefore may be potential regulators of systemic acid-base balance.