ENHANCEMENT OF METHIONINE PRODUCTION BY METHIONINE ANALOG ETHIONINE RESISTANT MUTANTS OF BREVIBACTERIUM-HEALI

Authors
MONDAL S CHATTERJEE SP
Citation
S. Mondal et Sp. Chatterjee, ENHANCEMENT OF METHIONINE PRODUCTION BY METHIONINE ANALOG ETHIONINE RESISTANT MUTANTS OF BREVIBACTERIUM-HEALI, Acta biotechnologica, 14(2), 1994, pp. 199-204
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Acta biotechnologica → ACNP
ISSN journal
01384988
Volume
14
Issue
2
Year of publication
1994
Pages
199 - 204
Database
ISI
SICI code
0138-4988(1994)14:2<199:EOMPBM>2.0.ZU;2-Y
Abstract
In order to improve the methionine yield of the isolate B. heali, atte mpts were made to isolate mutants resistant to the methionine analogue DL-ethionine after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanid ine (NTG). The minimum inhibitory concentration (MIC) of ethionine for B. heali was found to be 2 mM. After mutagenesis and screening, five mutants resistant to 50 mM of ethionine were isolated. The yield of th e best ethionine resistant mutant, B. heali Br EthR2(50), was 13 mg/l of methionine medium under optimum cultivation conditions.