Recent advances in the development and application of affinity biosens
ors are presented in this review. Current assay technology for the det
ection of ligand binding to antibodies, receptors, DNA, and other bind
ing proteins and selected approaches to the development of reversible,
non-destructive affinity biosensors are discussed. The problems to be
overcome for the commercialization of practical affinity biosensors a
re examined and future trends in affinity biosensor research are predi
cted.