LOCALIZATION OF A KINESIN-RELATED PROTEIN TO THE CENTRAL PAIR APPARATUS OF THE CHLAMYDOMONAS-REINHARDTII FLAGELLUM

Affinity-purified polyclonal antibodies raised against the conserved m otor domain of Drosophila kinesin (alpha HD) recognized a 110 kDa comp onent of the Chlamydomonas flagellar axoneme. Whole-mount immunogold l abeling of splayed axonemes showed the striking localization of this a ntigen along one of the two microtubules of the central pair apparatus . Interestingly, the alpha HD antigen was also localized along the cen tral axis of mutant axonemes lacking the central pair microtubules. Th ese results suggest that a 110 kDa kinesin-related protein is a struct ural component of the flagellar central pair apparatus and that it is correctly targeted even in the absence of the central pair microtubule s.