Cows that were subjected to hot environmental temperatures yielded les
s milk (3.1 kg/d) on a diet high in CP (18.4%) and of medium degradabi
lity (65%) than on diets high in CP of low degradability (59%) or medi
um in CP (16.1%). The high CP diets were associated with decreased DMI
and higher water intake, ruminal NH4, and blood urea. Negative effect
s on yield from the high CP, medium degradability diet were not observ
ed at moderate temperatures. Evaporative cooling of cows in hot weathe
r resulted in a greater milk yield response to low versus medium rumen
-undegradabale protein diets than for uncooled cows. Evaporative cooli
ng of cows also affected response to protein quality. For cooled cows,
high Lys diet (soybean, fish, and blood meals) increased milk yield 1
4% over that with low Lys diet (com gluten meal), but, for uncooled co
ws, a high Lys diet only increased yield by 9%. Percentage of CP, degr
adability, and protein quality had no effect on body temperatures or r
espiration rates of lactating cows. Some, but not other, reports showe
d that supplementation of 2 to 2.5% fat to diets fed under hot summer
conditions resulted in less yield response than when fat was added at
moderate temperatures. In several studies, fungal cultures (3 to 5 g/d
) in the diet decreased body temperatures and respiration rates in hot
, but not cool, weather. Increased milk yields and cellulose digestibi
lity also resulted from dietary fungal cultures in some, but not all,
trials. The mechanism of action exerted by fungal cultures on body tem
perature and respiration rate is unclear.