SUBSTRATE-SPECIFICITY OF PROLINE 4-HYDROXYLASE - CHEMICAL AND ENZYMATIC-SYNTHESIS OF 2S,3R,4S-EPOXYPROLINE

Authors
BALDWIN JE FIELD RA LAWRENCE CC LEE V ROBINSON JK SCHOFIELD CJ
Citation
Je. Baldwin et al., SUBSTRATE-SPECIFICITY OF PROLINE 4-HYDROXYLASE - CHEMICAL AND ENZYMATIC-SYNTHESIS OF 2S,3R,4S-EPOXYPROLINE, Tetrahedron letters, 35(26), 1994, pp. 4649-4652
Citations number
31
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Tetrahedron letters → ACNP
ISSN journal
00404039
Volume
35
Issue
26
Year of publication
1994
Pages
4649 - 4652
Database
ISI
SICI code
0040-4039(1994)35:26<4649:SOP4-C>2.0.ZU;2-V
Abstract
The substrate specificity of L-proline 4-hydroxylase, a 2-oxoglutarate dependent dioxygenase from Streptomyces griseoviridus P8648, was inve stigated. Preliminary assays measuring turnover of 2-oxoglutarate indi cated 3,4-dehydro-L-proline was an efficient substrate. The identity o f the product was shown to be 2S,3R,4S-epoxy-L-proline by comparison w ith synthetic 2S,3R,4S- and 2S,3S,4R-epoxy-L-prolines.